关键词: 免疫缺陷小鼠, 血液学, 淋巴细胞亚群, 脏器系数

Abstract: Objective　To clarify the biological characteristics of commercialized immunodeficient mice in China and provide a scientific basis for their standardized management and quality control.Methods Fourteen strains of immunodeficient mice from seven domestic enterprises were selected, which were classified into three categories, T cell-deficient, T and B cell-deficient, and T, B and NK cell-deficient. Their organ coefficients, hematological indicators, peripheral blood lymphocyte subsets, and histological characteristics of immune organs were detected and compared with those of age-matched BALB/c mice. Results　All three types of immunodeficient mice exhibited phenotypic characteristics corresponding to the deficiency of target immune cells. The thymus coefficients were significantly lower than those of control group, lymphocyte-related indicators (LYMPH, LYMPH%, CD3+ T cells, etc.) were significantly downregulated, and indicators such as neutrophil percentage (NEUT%) were significantly upregulated. However, statistical differences were observed in some hematological and lymphocyte subset indicators among mice of the same strain from different enterprises (P<0.05, P<0.01).Conclusion The overall quality of commercialized immunodeficient mice in China is stable, and their biological characteristics are basically consistent with those of immunodeficient mice. However, significant variations in biological indicators within certain strains deviate greatly from the average, indicating that standardization needs to be further improved.

Key words: immunodeficient mice, hematology, lymphocyte subsets, organ coefficients